LongAmpTaqPCR Kit E5200S from New England Biolabs

Product Specs
ItemLongAmpTaqPCR Kit
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE5200S
Quantity100 reactions
ApplicationsDNA Analysis, PCR
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Exome and genome sequencing to unravel the precise breakpoints of partial trisomy 6q and partial Monosomy 2qBMC PediatricsNovember 22, 2023Shuang Zhang, Qianwei Cui, Shangying Yang, Fangxia Zhang, Chunxia Li, Xiaoguang Wang, Bo Lei, Xunlun Shengaccording to the breakpoint regions of SVs (Table 1). PCR was carried out using a LongAmp Taq PCR Kit (E5200S; NEB, Ipswish, MA). A total of 50–100 ng of gDNA was used as the PCR template (conditions: 96 °C for

(2) Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United StatesPoultry ScienceAugust 1, 2023Carolina Trujillo-Peralta, Aaron Ashcraft, Roberto Señas-Cuesta, Makenly Coles, Xochitl Hernandez-Velasco, Callie Selby, Aaron Forga, Guillermo Tellez-Isaias, Christine Vuong, Lisa Bielke, et al.gene, a 1,272-base pair (bp) fragment, as described by Imai and Barta (2019). LongAmp Taq PCR kit (#E5200S New England Biolabs, Ipswich, MA) was used for PCR per the manufacturer's instructions. Magnesium sulfate

(3) Characterization of a Novel Thermostable DNA Lyase Used To Prepare DNA for Next-Generation SequencingChemical research in toxicologyFebruary 20, 2023Baljinnyam T, Conrad JW, Sowers ML, Chang-Gu B, Herring JL, Hackfeld LC, Zhang K, Sowers LC.each fluorescent primer (oligos 1 and 6 from Figure S15), 300 μM dNTPs, 5 units LongAmp Taq (NEB, #E5200S), and 1 μL template DNA in 25 μL of LongAmp buffer. Reactions were placed into a thermocycler preheated

(4) Removal of a 10-kb Gret1 transposon from VvMybA1 of Vitis vinifera cv. ChardonnayHorticulture ResearchSeptember 6, 2022Yingzhen Yang, John Ke, Xiaoyan Han, Wegi A Wuddineh, Guo-qing Song, Gan-Yuan Zhongshoots were used for RNA extraction and RT-PCR. LongAmp genomic PCR We used LongAmp® Taq PCR kit (NEB, E5200S) to amplify the MybA1 locus. Ten to fifty nanograms of grape genomic DNA extracted with Qiagen DNeasy

(5) Optical Genomic Mapping Identified a Heterozygous Structural Variant in NCF2 Related to Chronic Granulomatous DiseaseJournal of Clinical ImmunologyJuly 28, 2022Xiaoying Hui, Jingmin Yang, Jing Zhang, Jinqiao Sun, Xiaochuan Wangchr1:183,557,412–183,565,181 (length 7770 bp, with the speculated deletion) were first amplified using LongAmp Taq PCR Kit (E5200S; NEB, Ipswich, MA). Primer pairs and PCR conditions were LF: 5′-TAAAAAAGATAGGGAAATAGGAGAAGC-3′, and

(6) Concerted evolution reveals co-adapted amino acid substitutions in Na+K+-ATPase of frogs that prey on toxic toadsCurrent BiologyJune 1, 2021Shabnam Mohammadi et al.,Thermo Fisher Scientific™,Cat#451641 Agencourt DNAdvance Kit,"Beckman Coulter, France",Cat#A48705 LongAmp® Taq PCR Kit,NEB,Cat#E5200S Ligation Sequencing Kit,Oxford Nanopore Technology,SQK-LSK109 Deposited Data,Deposited Data,Deposited

(7) Potential role for age as a modulator of oral nitrate reductase activityNitric OxideMarch 1, 2021Khandaker Ahtesham Ahmed et al.from tongue scrapes. Extracted DNA was used as a template for PCR (LongAmp Taq PCR kit (NEB, cat. no. E5200S) using unique barcoded primers to V4 region of the 16S rRNA gene to generate an amplicon library for

(8) CROT (Carnitine O-Octanoyltransferase) Is a Novel Contributing Factor in Vascular Calcification via Promoting Fatty Acid Metabolism and Mitochondrial DysfunctionArteriosclerosis, thrombosis, and vascular biologyFebruary 1, 2021Okui T, Iwashita M, Rogers MA, Halu A, Atkins SK, Kuraoka S, Abdelhamid I, Higashi H, Ramsaroop A, Aikawa M, et al.primers (see Supplemental Major Resources Table) and LongAmp® Taq PCR Kit (New England Biolabs., Cat#: E5200S) according to manufacturer’s protocol. In vivo vascular calcification imaging 10-week-old male Cro+/+Ldlr−/−

(9) Blood and lymphatic systems are segregated by the FLCN tumor suppressorNature communicationsDecember 9, 2020Tai-Nagara I, Hasumi Y, Kusumoto D, Hasumi H, Okabe K, Ando T, Matsuzaki F, Itoh F, Saya H, Liu C, et al.confirming correct homologous recombination were carried out using a LongAmp Taq PCR Kit (NEB, Cat# E5200S), according to the manufacturer’s protocol. The mice can be obtained from Dr. Yoh-suke Mukouyama (mukoyamay@nhlbi.nih.gov). Preparation

(10) Mitochondrial DNA Stress Signalling Protects the Nuclear GenomeNature metabolismDecember 1, 2019Wu Z, Oeck S, West AP, Mangalhara KC, Sainz AG, Newman LE, Zhang XO, Wu L, Yan Q, Bosenberg M, et al.GeneJET genomic DNA purification kit (Thermo Scientific, #K0721) and LongAmp Tag PCR kit (New England Biolabs, #E5200S). PCR primers (Supplemental Table 1) siRNAs (Supplemental Table 2) and gRNAs (Supplemental Table 3)

(11) Long‐fragment targeted capture for long‐read sequencing of plastomesApplications in Plant SciencesMay 8, 2019Kevin Bethune et al.et al. (2016) for P. barteri (Appendix S1). LR‐PCR was carried out using the LongAmp Taq PCR kit (#E5200S; New England BioLabs, Ipswich, Massachusetts, USA) following the manufacturer's instructions in a final

(12) The Purple Sea Urchin Strongylocentrotus purpuratus Demonstrates a Compartmentalization of Gut Bacterial Microbiota, Predictive Functional Attributes, and Taxonomic Co-OccurrenceMicroorganismsJanuary 26, 2019Joseph A. Hakim, Julie B. Schram, Aaron W. E. Galloway, Casey D. Morrow, Michael R. Crowley, Stephen A. Watts, Asim K. Bejamplification was performed using the LongAmp Taq PCR Kit (New England Biolabs, Ipswich, MA, USA; catalog no. E5200S) at a total reaction volume of 50 µL with the following reagents: 10 µL of 5× Reaction Buffer; 1.5 µL of

(13) Ocean warming alters predicted microbiome functionality in a common sea urchinProceedings of the Royal Society B: Biological SciencesJune 27, 2018Cecilia J. Brothers, William J. Van Der Pol, Casey D. Morrow, Joseph A. Hakim, Hyunmin Koo, James B. McClintockAL, USA]) [10,11,26]. PCR products were obtained using New England Biolabs LongAmp Taq PCR kits (Cat# E5200S), electrophoresed on an agarose gel, and visualized by UV illumination [26]. The PCR product was excised

(14) Gut microbiome diversity influenced more by the Westernized dietary regime than the body mass index as assessed using effect size statisticMicrobiologyOpenAugust 1, 2017Shannon C. Davis, Jagjit S. Yadav, Stephanie D. Barrow, Boakai K. RobertsonAttachment 1). 3.2 16S rRNA gene polymerase chain reaction (16S PCR) The NEB LongAmp Taq PCR kit (Cat# E5200S) was used to carry out the PCR reactions. The unique degenerate barcoded primers used in the PCR reaction

(15) Cross-fostering immediately after birth induces a permanent microbiota shift that is shaped by the nursing motherMicrobiomeDecember 1, 2015Joseph G Daft, Travis Ptacek, Ranjit Kumar, Casey Morrow, Robin G Lorenzof the dNTPs; 2 μL (1.5 μM) of each of the primers; 1.5 μL (5 U) of the ‘LongAmp’ enzyme kit (cat # E5200S; New England Biolabs, Ipswich, MA, USA); 30 μL 2 to 5 ng/ μL of the Template DNA prepared using the

(16) Vaginal Microbiota in Pregnancy: Evaluation Based on Vaginal Flora, Birth Outcome, and RaceAmerican Journal of PerinatologyOctober 20, 2015Ranjit Subramaniam, Suzanne Cliver, Suzanne P. Cliver, Degui Szychowski, Jeff M. Abramovici, Joseph Abramovici, Joseph R. Lefkowitz, Elliot J. Lefkowitz, Rodney Edwards, Rodney K. SubramaniamμL of the “LongAmp” enzyme kit (New England Biolabs [Ipswich, MA] LongAmp Taq PCR kit; catalog no. E5200S), 30 μL of the template DNA (prepared using the fecal DNA isolation kit with the concentration of DNA

(17) An abundance of Epsilonproteobacteria revealed in the gut microbiome of the laboratory cultured sea urchin, Lytechinus variegatusFrontiers in MicrobiologyOctober 13, 2015Joseph A. Hakim, Hyunmin Koo, Lacey N. Dennis, Ranjit Kumar, Travis Ptacek, Casey D. Morrow, Elliot J. Lefkowitz, Mickie L. Powell, Asim K. Bej, Stephen A. Wattsoligonucleotide primers; 1.5 µL (5 U) of the “LongAmp” enzyme kit (New England Biolabs, Ipswich, MA; cat # E5200S); 30 µL (2–5 ng/µl) of the template DNA; and 3 µL of sterile H2 O to a total reaction volume of 50 µL.

(18) Getting Started with Microbiome Analysis: Sample Acquisition to BioinformaticsCurrent Protocols in Human GeneticsJuly 14, 2014Ranjit Kumar, Peter Eipers, Rebecca B. Little, Michael Crowley, David K. Crossman, Elliot J. Lefkowitz, Casey D. Morrow10μM for use in PCR reactions). New England Biolabs LongAmp Taq PCR kit (http://www.neb.com, cat # E5200S). The kit contains all of the necessary reagents for the PCR reactions. Qiagen QIAquick Gel Extraction

(19) VlincRNAs controlled by retroviral elements are a hallmark of pluripotency and cancerGenome BiologyJuly 22, 2013Georges St Laurent et al.RNAse H, incubated at 37 °C for 30 minutes. Subsequent PCR was performed using Long Amp Taq PCR Kit (NEB, E5200S) following the manufacturer's protocol on 2.5 µl cDNA in a 25 µl final volume (94 °C 30s, 34x(94 °C

(20) Intronic RNAs constitute the major fraction of the non-coding RNA in mammalian cellsBMC GenomicsSeptember 24, 2012Georges St Laurent, Dmitry Shtokalo, Michael R Tackett, Zhaoqing Yang, Tatyana Eremina, Claes Wahlestedt, Silvio Urcuqui-Inchima, Bernd Seilheimer, Timothy A McCaffrey, Philipp KapranovRnase H, incubated at 37°C for 30 minutes. Subsequent PCR was performed using Long Amp Taq PCR Kit (NEB, E5200S) following the manufacturer’s protocol on 2.5ul cDNA in a 25ul final volume (94°C 30s, 34x (94°C 30

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(1)

2/3/2020Amplification of 25 Kb DNA Insert from Low-Complexity and Difficult Templates from Human Genomic DNADr. Thippeswamy SannasiddappaIdeal PCR kit in the molecular biology labs if one is intending to amplify long DNA inserts from low complexity and difficulty amplify DNA templates like human or eukaryotic DNA samples.
(1)

9/27/2019Exceptional Performance with Long Amplicons Atul SinghHighly recommend for PCR amplification of more than 10kb.

LongAmpTaqPCR Kit

LongAmpTaqPCR Kit from New England Biolabs

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New England Biolabs

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LongAmpTaqPCR Kit from New England Biolabs

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